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. 2017 Dec 14;9(5):6015–6027. doi: 10.18632/oncotarget.23210

Figure 4. CD8 T cells utilize Fas/FasL to kill E7 expressing KC.

Figure 4

Co-cultures of effector cells and SIINFEKL-loaded KC were treated with inhibitory antibody to Fas (A-D) or FasL (E-H) or isotype control antibodies (iso). (A, E) Death of KC in co-culture (black) or monoculture controls (grey). (B, F) Survival kinetics of KC-effector co-cultures. (C, G) Duration of effector-KC attachment. (D, H) T cell death rate. Freshly-derived primary KC were examined for Fas (I) or FasL (J) expression by flow cytometry. Gating delineated living cells that were CD45 negative. Each value represents one mouse. (K, M) Confocal micrograph (maximal intensity projection) of primary KC cultured for 5 days and stained for surface expression of Fas (green) (K) or FasL (red) (M). (L, N) Primary KC were cultured for 5 days and cell lysates were harvested for RNA extraction. RT-PCR for Fas (L) or FasL (N) mRNA was performed comparing expression to a house-keeping gene. Each value represents one mouse. (*p<0.05; n.s not significant; bar is 50μm).