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. 2017 Dec 14;9(5):6015–6027. doi: 10.18632/oncotarget.23210

Figure 5. Increased levels of PD-L1 expression by E7KC is protective.

Figure 5

Co-cultures of CTL and SIINFEKL-loaded B6KC or E7KC were treated with anti-PD-L1 antibody or isotype control antibody. (A) Death of KC in co-culture with effectors (black) or as monocultures (grey), or in co-cultures without PD-L1 inhibition (white). (B) CTL-target attachment duration. (C) CTL death in co-cultures. (D) Flow cytometry of primary KC harvested as in Figure 4E, and stained for PD-L1 expression using the same gating strategy. Isotype control is also shown. (E) Primary KC were cultured for 5 days and cell lysates were harvested for RNA extraction and subject to RT-PCR for PD-L1 mRNA quantification. Each value is one mouse. (*p<0.05).