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. 2017 Dec 14;9(5):6015–6027. doi: 10.18632/oncotarget.23210

Figure 6. Hierarchical killing of KC by CTL is altered by E7 expression.

Figure 6

T cell killing mechanism hierarchy was investigated by inhibition of multiple pathways during co-culture of primary non-transgenic KC (B6) or E7-expressing KC (E7) loaded with constant amount of peptide and perforin-competent or Prf1-/- effector CD8 T cells. Co-cultures were incubated with anti-Fas (A), or anti-FasL (B) inhibitory antibody. (C) Co-cultures of effector and target cells were incubated with anti-PD-L1 inhibitory antibody, with or without Fas inhibition. (D) Co-cultures were treated with anti-TNFα antibody with or without Fas inhibition. (E) Co-cultures were treated with anti-IFNγ with, or without, Fas inhibition. Average values of five imaging sites of duplicate wells for each experiment are shown as one dot. (*p<0.05) Data from single inhibitor experiments Figure 2-5 have been reshown in these graphs for ease of comparison. Note that the ‘+’ indicates the functional presence of the particular cytokine while ‘–’ indicates its absence by knock-out or by antibody (e.g. If Fas is present, there is a ‘+’, while Fas inhibition with antibody is indicated by ‘–’).