Figure 3. LIMD1 mRNA level is associated with EBV latency and upregulated by LMP1.

(A) RNA was extracted from indicated different pair of cell lines, and LIMD1 mRNA expression level was evaluated by real-time qPCR. The average mRNA levels of the duplicates in SavI, BJAB, P3HR1, and CEM were set to 1. (B) Cell lines with high endogenous NFκB activity were treated with 2.5 µM Bay11-7085 for 48 h. RNA was then extracted for real-time qPCR analysis for LIMD1 expression. The average LIMD1 mRNA levels of the duplicates in DMSO-treated cells were set to 100%. The LIMD1 mRNA levels decreased by Bay11-7085 treatment are shown as percentage of those with corresponding DMSO controls. Statistical analysis was performed on results from three independent experiments.