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. 2017 Dec 26;9(5):6282–6297. doi: 10.18632/oncotarget.23676

Figure 5. LIMD1 is required for LMP1 signal transduction and regulation of target genes.

Figure 5

(A) and (B) IB4 cell lines stably expressing LIMD1 shRNA #1 and #2 were induced by 1 µg/ml doxycycline for 48 h for LIMD1 shRNA expression, and cell lysates were then subjected to immunoblotting for analysis of the LMP1 downstream pathway activity and target gene expression. (C) LIMD1, IRF4, p62 and Bcl6 in IB4 stable cell line expressing LIMD1 shRNA #1 were also analyzed at mRNA levels using real-time quantitative PCR. The average mRNA levels of the duplicates in shControl-expressing cells were set to 100%.