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. 2017 Dec 26;9(5):6282–6297. doi: 10.18632/oncotarget.23676

Figure 6. LIMD1 depletion potentiates ionomycin-induced DNA damage and apoptosis, and impairs autophagy.

Figure 6

(A) IB4 cell lines stably expressing LIMD1 shRNA #1 and control were treated with ionomycin for 48 h, and apoptosis was analyzed by flow cytometry for Annexin V binding. The right graph shows an analysis for a representative experiment with duplicate for each sample (mean ± SE). (B–D) IB4 cell lines (B) and (D) and JiJoye cell lines (C) stably expressing LIMD1 shRNA #1 and control were treated with ionomycin for 48 h (B) and (D) or time points (C). 2.5 µg/ml and 5.0 µg/ml in (B) and 2.5 mg/ml in (C) and (D). DNA damage, apoptosis, and autophagy were evaluated by immunoblotting for related hallmarks: the DNA damage hallmark γH2AX, the autophagy hallmark LC3-II, and the apoptosis hallmark Caspase 3 activity. Bim and Noxa were also analyzed.