Figure 5. Interrogation of publically available datasets also indicates a stronger fit to the neutral evolution model in untreated primary tumors.

(A) Boxplot of the R² goodness-of-fit for the neutral evolution model, presenting data from cohort 2. The minimum purity level of included samples is categorized below the x-axis and the 93 samples which satisfied inclusion criteria for analysis using purity-adjusted VAFs are compared. The line of short dashes indicates the R² = 0.98 threshold used to distinguish neutral and non-neutral evolution and the number of samples greater than this threshold out of the total number analyzed in each category is indicated above the dashed line. (B) Boxplot of the R² goodness-of-fit for the neutral evolution model, presenting data from cohort 3. The line of short dashes indicates the R² = 0.98 threshold. A two-way analysis of variance was performed using the general linear model for continuous variables and the corresponding p-value is displayed.