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. 2018 Jan 3;9(8):7996–8010. doi: 10.18632/oncotarget.23847

Figure 2. Effect of SIRT1 on binucleation.

Figure 2

(A) H9c2 cells were treated in the absence or presence of SIRT1 inhibitor nicotinamide (NAM; 10 mM) for 48 hours and binucleation was induced by retinoic acid for 5 days. Data are means ± SEM. n = 5 *P < 0.05, RA + NAM versus RA. (B) H9c2 cell were transfected with 50 nM SIRT1 siRNAs or 50 nM siRNA negative controls (NC) for 48 hours. SIRT1 protein and mRNA abundance was determined with Western immunoblotting or RT-PCR. Data are the means ± SEM. n = 4 *P < 0.05, siRNA vs. NC. (C) H9c2 cells were transfected with SIRT1 siRNAs or negative controls (NC) for 48 hours and binucleation was induced by retinoic acid for 5 days. Data are the means ± SEM. n = 5 *P < 0.05, RA + siRNA vs. RA + NC.