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. 2018 Jan 3;9(8):7996–8010. doi: 10.18632/oncotarget.23847

Figure 5. MiR133a binds to SIRT1 mRNA 3′UTR and inhibits SIRT1 protein expression.

Figure 5

(A) SIRT1 mRNA 3′UTR shows complementary binding sequences to the seed sequences of rat miR133a. H9c2 cells were transfected with pmiRGLO vector containing putative miR133a binding sites within the SIRT1 3′UTR (pmiRGLO-SIRT1) and co-transfected with miR133a mimic or negative control scramble for 48 hours. Luciferase activity was measured. Data are mean ± SEM. n = 6 *P < 0.05, miR133a mimic versus pmiRGLO-SIRT1 alone. (B) SIRT1 protein abundance was measured in H9c2 cells transfected with miR133a mimic or negative control (NC) for 48 hour. Data are mean ± SEM. n = 4 *P < 0.05, miR133a mimic versus negative control.