Skip to main content
. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Int J Cancer. 2017 Dec 15;142(8):1652–1663. doi: 10.1002/ijc.31196

Figure 2. Ezrin mediates the regulation of autocrine Met signaling-induced metastasis.

Figure 2

(a) Western blot analysis of B16F1-HGF cells transfected with vector (shc), Ezrin shRNA (sh1, sh2) expressing constructs. sh1-1, sh1-2 are two cell clones from sh1 construct, sh2-1 and sh2-2 are two cell clones from sh2 construct. (b–e) B16F1-HGF cells transfected with vector (shc) and Ezrin shRNAs expressing constructs described in (a) were analyzed with CCK8 to assess cell proliferation (b), with a transwell assay to determine cell motility (c), with a matrigel-coated transwell assay to determine cell invasiveness (d) and with an experimental metastatic assay by tail vein injection to determine gross pulmonary metastasis (e) (shc, n=10; sh1-1, n=9; sh1-2, n=8; sh2-1, n=9; sh2-2, n=8). (f) Western blot analysis of B16F1-HGF/Met cells transfected with vector (shc), Ezrin shRNA (sh1, sh2) expressing constructs. sh1-1, sh1-2 are two cell clones from sh1 construct, sh2-1 and sh2-2 are two cell clones from sh2 construct. (g–l) B16F1-HGF/Met cells transfected with vector (shc) and Ezrin shRNAs expressing constructs described in (f) were analyzed with CCK8 to assess cell proliferation (g), with a transwell assay to determine cell motility (h), with a matrigel-coated transwell assay to determine cell invasiveness (i), with an experimental metastatic assay by tail vein injection to determine gross pulmonary metastasis (j) (shc, n=8; sh1-1, n=9; sh1-2, n=9; sh2-1, n=9; sh2-2, n=9), with representation of lung H&E staining of metastases (k), and with macro- and micro- metastases counted in lung sections (l) (shc, n=6; sh1-2, n=6; sh2-2, n=6).