Extended Data Figure 9. Stat3 and TGFβ signal synergistically to promote c-Maf expression.

a, Above, representative flow cytometry plots depicting RORγt and Foxp3 expression in CD4⁺ T cells in the LILP of CD4-dnTGFbRII and littermate controls (n=3). Below, representative plot of c-Maf expression in Foxp3⁺ cells from above animals. b, Above, representative flow cytometry plots depicting RORγt and Foxp3 expression in CD4⁺ T cells in the LILP of Stat3^fl/fl; CD4^Cre and Stat3^fl/fl littermate controls (n=4). Below, representative plot of c-Maf staining in Foxp3⁺ cells from above animals. c, Mean fluorescence intensity of c-Maf staining in in vitro differentiated CD4⁺ T cells. Naïve CD4⁺ T cells from WT, Stat3^fl/fl;CD4^Cre and Maf^fl/fl;CD4^cre mice were activated for 48 h with α-CD3ε/α-CD28 under indicated conditions. Dashed line represents the MFI of c-Maf in Maf^fl/fl;CD4^cre T cells. Data shows one of two independent experiments.