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. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Nature. 2018 Feb 7;554(7692):373–377. doi: 10.1038/nature25500

Figure 2. H. hepaticus predominantly induces inflammatory TH17 cells in IL-10 deficiency-dependent colitis.

Figure 2

a–d, LILP HH7-2tg and SILP 7B8tg donor-derived cells in Il10+/− (n=8) and Il10−/− (n=10) mice were analyzed for Foxp3 and RORγt expression (a), frequencies of Treg (Foxp3+) and TH17 (Foxp3−RORγt+) (b), RORγt and T-bet co-expression (c), and frequencies of T-bet expression among TH17 (Foxp3−RORγt+) cells (d). Data are from four independent experiments. e, f, IL-17A and IFNγ expression (e) and frequencies of IL-10, IL-17A and IFNγ positive cells (f) among LILP HH7-2tg donor-derived cells in Il10+/− (n=5) and Il10−/− (n=6) mice after re-stimulation. Data summarize two independent experiments. All statistics were calculated by unpaired two-sided Welch’s t-test. Error bars: mean ± 1 SD. P values are as follows: b, i=9.48x10−12 and ii=1.11x10−13. d, i=2.16x10−9, ii=1.22x10−10 and iii=0.36.