Extended Data Figure 1. Cloning and characterization of H. hepaticus-specific TH17 TCRs, and generation of TCR transgenic (TCRtg) mice and MHC-II tetramers.
a, IL-23R-GFP expression in CD4+ T cells from the large intestines of mice with and without H. hepaticus colonization and after IL-10Ra blockade. Data are from one of five independent experiments. b, Experimental scheme for cloning H. hepaticus-induced single IL-23R-GFP+ (predominantly TH17) cell TCRs under IL-10Ra blockade. c, Summary of the twelve dominant H. hepaticus-induced TH17 TCRs. d, In vitro activation of CFSE-labeled naive HH7-2tg and HH5-1tg cells by indicated stimuli in the presence of antigen-presenting cells. Data are from one of two independent experiments. e, f, Expansion of donor-derived HH7-2tg (e) and HH5-1tg (f) (CD45.2) cells in the large intestine (LI) of H. hepaticus-colonized or -free CD45.1 mice, gated on total CD4+ T cells. Data are from one of three independent experiments. g, HH-E2 tetramer staining of CD4+ T cells from the LI of H. hepaticus-colonized or -free mice. Data are from one of six independent experiments.