Table 2.

List of novel miRNAs discoveries and their information

ID	miRNA	Length of Precursor	ID transcript	% A and U	ΔG	AMFE	MFEI
Pmi-nov_1	ATGGATATGCAAGCATGATA	133	comp66662_c8_seq2	66.92	− 37.1	27.89	1.19
Pmi-nov_2	CGGGGAAGAGGCTGAGCAAGGA	103	comp64725_c0_seq1	50.49	− 57.5	55.83	0.89
Pmi-nov_3	CTGTTAGCAGTAGTACAATTA	133	comp60703_c0_seq2	59.40	− 55.1	41.43	0.98
Pmi-nov_4	CTTGATTGAAATGGTTCTCGACGA	83	comp52499_c0_seq1	60.24	− 24.6	29.64	1.34
Pmi-nov_5	GGAGCGACCTTAGACCACATGA	163	comp6546_c0_seq1	51.53	− 59.0	36.20	1.34
Pmi-nov_6	GTGCTCTCTCTCATTGTCATA	113	comp51053_c0_seq1	55.75	− 58.0	51.33	0.86
Pmi-nov_7	TCCCACTCTCAACACCAA	83	comp62823_c0_seq1	44.58	− 28.6	34.46	1.61
Pmi-nov_8	AATGTATTTTTGGACGGAGGTAGTA	82	comp66383_c1_seq1	67.07	− 13.8	16.83	1.96
Pmi-nov_9	GTGCTCTCTCTCGTTGTCA	143	comp7042_c0_seq1	59.44	− 61.6	43.07	0.94
Pmi-nov_10	ACGGCTGCTCGATGACCA	113	comp51416_c0_seq1	48.67	− 42.7	37.79	1.36
Pmi-nov_11	AGGTCACAAATGGACGGTTGA	113	comp59558_c0_seq1	48.67	− 57.9	51.24	1.00

From left, miRNA candidate, novel mature miRNA sequence, length of secondary structure, ID transcript that used for secondary structure prediction, percentage (%) of A and U base, MFE (ΔG), AMFE and MFEI value