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. 2018 Feb 15;8:3079. doi: 10.1038/s41598-018-21340-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy and DAPI staining confirm the role of domains B and C in nuclear targeting of B1 in GF-1 cells. (a) Phase-contrast fluorescence images showing the distribution of EYFP, EYFP-B1 and different B1 domain deletion mutants in transfected GF–1 cells at 48 hpt. Panels a1–a4 show EYFP distributed in the cytoplasm; panels b1–b4 show EYFP-B1 distributed in the nucleus; panels c1–c4 show EYFP-B1ΔA distributed in the nucleus; panels d1–d4 show EYFP–B1ΔB distributed in the cytoplasm (major) and nucleus (minor; indicated by arrows); panels e1–e4 show EYFP–B1ΔC distributed in the cytoplasm (indicated by arrows); panels f1–f4 show EYFP–B1ΔD distributed in the nucleus; panels g1–g4 show EYFP–B1ΔBC distributed in the cytoplasm; panels h1–h4 show EYFP–B1ΔAD distributed in the nucleus. The cells were also traced with green fluorescence and DAPI nuclear staining and the final images were merged to confirm localization. Scale bar = 10 μM. (b) Comparison of different nuclear targeting signal sequences.