Table 2.
The RGNNV B1 sequence primers used in this study.
| Name | Primers* | |
|---|---|---|
| pEYFP-B1ΔA | Forward 1 | AGCTAAGCTTCGATGTCTGTCGATTAGAC (HindIII) |
| Reverse 1 | AATTGAATTCAGGTGCTGATCGATCGCT (EcoRI) | |
| Forward 2 | AATTGAATTCGCAGCTCTTCCAGGTGAT (EcoRI) | |
| Reverse 2 | GATCGGATCCCTACACTTGAGTGCGACG (Stop) | |
| pEYFP-B1ΔB | Forward 1 | AGCTAAGCTTCGATGTCTGTCGATTAGAC (HindIII) |
| Reverse 1 | AATTGAATTCCGCGTGTCCATCATCACC (EcoRI) | |
| Forward 2 | AATTGAATTCAGTGACCGCGATCCAGGT (EcoRI) | |
| Reverse 2 | GATCGGATCCCTACACTTGAGTGCGACG (Stop) | |
| pEYFP-B1ΔC | Forward 1 | AGCTAAGCTTCGATGTCTGTCGATTAGAC (HindIII) |
| Reverse 1 | AATTGAATTCAACGTGCGCATCTCGTTT (EcoRI) | |
| Forward 2 | AATTGAATTCAGCTCGCCGCCTGCACGT (EcoRI) | |
| Reverse 2 | GATCGGATCCCTACACTTGAGTGCGACG (Stop) | |
| pEYFP-B1ΔD | Forward 1 | AGCTAAGCTTCGATGTCTGTCGATTAGAC (HingIII) |
| Reverse 1 | GATCGGATCCCTACACAGCAGCTCGGCCTAG (Stop) | |
| pEYFP-B1ΔBC | Forward 1 | AGCTAAGCTTCGATGTCTGTCGATTAGAC (HindIII) |
| Reverse 1 | AATTGAATTCCGCGTGTCCATCATCACC (EcoRI) | |
| Forward 2 | AATTGAATTCAGCTCGCCGCCTGCACGT (EcoRI) | |
| Reverse 2 | GATCGGATCCCTACACTTGAGTGCGACG (Stop) | |
| pEYFP-B1ΔAD | Forward 1 | AGCTAAGCTTCGATGTCTGTCGATTAGAC (HindIII) |
| Reverse 1 | AATTGAATTCAGGTGCTGATCGATCGCT (EcoRI) | |
| Forward 2 | AATTGAATTCGCAGCTCTTCCAGGTGAT (EcoRI) | |
| Reverse 2 | GATCGGATCCCTACACAGCAGCTCGGCCTAG (Stop) |
*The enzymatic cutting sites on nucleotides of primers are underlined.