Figure 1.

Experimental design and analysis pipeline. Five adult cynomolgus macaques were immunized, two months apart with MVA HIV-B at a dose of 4 × 10⁸ PFU injected subcutaneously. Blood was collected longitudinally at the indicated timepoints, hours (H) or days (D), post-prime (PP) and post-boost (PB), for Luminex, ELISA, and mass cytometry analyses to evaluate the plasma concentrations of cytokines and CRP and to phenotype in deep innate myeloid cells. Local inflammation was also scored at the indicated timepoints. Baseline samples were collected 21 and 19 days before the first vaccine injection for plasma soluble factors and single cell mass cytometry analysis, respectively, as well as just before the first immunization at H0PP. A blood draw was also collected just before the boost at D58PP/H0PB.