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. 2018 Feb 15;8:3081. doi: 10.1038/s41598-018-21361-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of SDHB induces the EMT pathway and enhances expression of glycolytic enzymes. (A) Western blotting of proteins involved in glycolysis and oxidative phosphorylation in SDHB-knockdown cells. Total proteins isolated from cells were hybridized with antibodies for glycolytic and aerobic respiration enzymes. (B) Western blot analysis of EMT-related proteins in SDHB-knockdown cells. Total proteins prepared from cells as indicated were blotted with antibodies against EMT-related proteins, including the indicated cell markers and transcriptional regulators. (C) Western blot analysis of HIF1α in SDHB-knockdown cells. Total proteins purified from cells were blotted with antibodies against SDHB, HIF1α and β-actin. The level of β-actin serves as a control for protein loading.