Fig. 1.

The Lk-GAL4 drives GFP expression in four distinct neuronal populations in the adult Drosophila CNS. a One pair of neurons in the lateral horn (lateral horn LK neurons; LHLKs) and another pair of neurons in the subesophageal ganglion (subesophageal ganglion LK neurons; SELKs) express LK in adult brain of Drosophila. Lk-GAL4 also drives weak and variable expression in four pairs of neurons in the brain (approximate location of these cells is indicated by the white box; see Figure S2 for an alternate preparation where these cells are weakly stained). b These four pairs of neurons do not display any LK-immunoreactivity, but are positive for ITP-immunoreactivity [21]. c In the ventral nerve cord (VNC), LK is expressed in 11 pairs of neurons (abdominal LK neurons; ABLKs). Seven pairs of smaller neurons in the posterior region (p) persist from the larval stages and the other four pairs (the number of pairs can vary between individuals) of larger neurons in the anterior region (a) are adult specific [48]. d A schematic depiction of LK-expressing neurons in the adult brain and VNC of Drosophila. T1–T3, thoracic neuromeres. e Lk-GAL4 also drives ectopic expression in the salivary glands of adult Drosophila. JFRC29-10xUAS-IVS-myr::GFP-p10 was utilized in a, c and e, whereas UAS-mcd8-GFP was utilized in b