Fig. 5.

Isolation of a novel and rare allele of TaGW2-A1. a 114-bp Insertion/Deletion (InDel) detected in the promoter region. Red rectangles specify the forward and reverse primers of TaGW2-A1_InDel. Blue and green rectangles indicate the transcription start site and the predicted translation initiation codon, respectively. b Comparison of the GUS expression level in different promoter-GUS constructs. Superscripts A, B, and C indicate the means (± SE) that significantly differ at the 0.01 level (LSD). c Relative expression levels of TaGW2-A1 in immature grains (11 dap) of the NIL1 pair, with Actin used as the endogenous control. The results of three biological replications showed similar trends. The values represent the means (± SE) of three biological replicates. *** indicates significance at the 0.001 level (Student’s t test). d Frequency of the 114-bp deletion among 1113 wheat accessions with varying ploidy. The two numbers (from left to right) within the brackets indicate the number of accessions amplifying the 224-bp fragment and the 336-bp fragment, respectively. e PCR products of TaGW2-A1_InDel in several hexaploid wheat accessions