Table 3.

Comparison of actual and predicted molecular weight on testing sets.

No.	Enzymatic temperature (°C)	Enzymatic Time (h)	Enzyme dose (U)	Substrate concentration (mg/mL)	Glucose concentration (mg/mL)	Actual molecular weight (kDa)	Predicted molecular weight (kDa)	APE (%)
1	25	5	8.10	4	0.33	417.31	422.04	1.13
2	25	9	8.10	4	0.36	329.91	324.8	1.55
3	25	1	12.15	6	0.27	710.67	706.96	0.52
4	25	4	12.15	6	0.68	388.68	376.18	3.22
5	25	8	12.15	6	0.72	289.12	296.91	2.70
6	25	9	12.15	6	0.69	272.03	264.98	2.59
7	25	10	8.10	8	1.00	381.42	388.45	1.84
8	25	11	12.15	10	1.10	329.91	325.73	1.27
9	25	7	12.15	4	0.48	286.61	281.84	1.66
10	25	11	8.10	12	1.12	404.20	415.75	2.86
11	30	10	9.72	4	0.22	200.88	200.01	0.43
12	30	11	9.72	4	0.20	194.57	187.06	3.86
13	35	5	9.72	4	0.30	249.35	245.92	1.38
14	35	10	8.10	4	0.30	227.90	233.83	2.60
15	35	8	8.10	4	0.36	250.08	249.81	0.11
16	35	4	8.10	4	0.41	330.87	340.43	2.89
17	35	6	9.72	12	0.76	413.10	425.79	3.07
18	35	11	12.15	6	0.73	261.96	257.18	1.82
19	35	10	12.15	4	0.46	241.17	247.2	2.50
20	25	9	9.72	4	0.16	224.62	225.07	0.20
21	25	10	12.15	8	0.63	268.50	268.05	0.17
22	35	11	12.15	6	0.36	214.74	209.3	2.53
MAPE (%)								1.86