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. 2018 Feb 15;8:3101. doi: 10.1038/s41598-018-21387-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Incubation of recombinant cytokines with RBCs. Boxplot summaries of cytokine log2 transformed raw fluorescence response data for RBC lysates collected using (a) the Bio-Plex human 27-plex panel and (b) the Bio-Plex human 21-plex panel (n = 5). Cells were incubated for 24 hours (37 °C) with or without recombinant cytokines (BP, and control, respectively). Data are statistically significantly different if p < 0.05 (*), or p < 0.001 (***).