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Cytokines in cell lysates and conditioned media following cell washing. Concentration of cytokines in the conditioned media of RBCs washed once (control RBCs) or ten times (washed RBCs) and subsequently incubated in PBS (400 × 106 cells per mL) for 24 hours at 37 °C as measured by Bio-Plex. Data are presented as mean with minimum and maximum values (n = 10). Data are significant if p < 0.05 (*), p < 0.01 (**), or p < 0.001 (***).
