Skip to main content
. 2018 Feb 15;8:3102. doi: 10.1038/s41598-018-21476-w

Table 1.

Kinetic parameters of 6MP and 6TG inhibition of human USP2 via deubiquitination assay.

USP2 in Structure Kis (μM) Kinact (μM)b
6 MPa graphic file with name 41598_2018_21476_Figa_HTML.gif 349.0 ± 23.7 —
6TGa graphic file with name 41598_2018_21476_Figb_HTML.gif 24.6 ± 1.8 15.6 ± 3.2
ML364c graphic file with name 41598_2018_21476_Figc_HTML.gif IC50: 1.1 μM

aIn the presence of 6MP or 6TG, the apparent kinetic parameters were determined from the best fit of the data to a noncompetitive inhibition model (Eq. 1). The Rsqr were 0.980 and 0.981 for that of 6MP and 6TG, respectively.

bThe Kinact was determined from the best fit of the data to the saturation equation.

cML364 is a USP2 inhibitor and the IC50 value was from Davis et al.6.