Table 1.
Stock fiber-bathing solutions
| Solution | pCa | IS | pH | ATP | BES | MOPS | EGTA | CaCl2 | MgCl2 | MgOAc | Na2CP | CPK |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Swank Relaxing | 8 | 200 | 7.0 | 12 | 20 | 5 | 0.1 | 14 | 30 | 600 | ||
| Reedy Relaxing | 9.67 | 100 | 6.8 | 15 | 20 | 5 | 15 | |||||
| Swank Activating | 4 | 200 | 7.0 | 12 | 20 | 5 | 5.5 | 14 | 30 | 600 | ||
| Reedy Activating | 4.5 | 100 | 6.8 | 15 | 20 | 5 | 5.0 | 15 |
All units are mM except CPK, which is U/ml. Ionic strength (IS) was kept constant between activating and relaxing solutions by using sodium methane sulfonate (NaMs) for Swank solutions, 6.9 mM in relaxing and 18.4 mM in activating, and by adding 10 mM NaCl to Reedy activating solution. Additionally, 1 mM DTT was added to both Swank solutions and 5 mM NaN3 to both Reedy solutions. pH was adjusted with KOH for Swank solutions and NaOH for Reedy solutions. Free calcium was calculated using either MaxChelator (3) or Calcium (15).