Table 3.
Species/Solution | ASA | PSA | FSA | Hill coefficient | pCa50 |
---|---|---|---|---|---|
Dros/Swank | 9.2 ± 0.8 [12]LS LR | 5.7 ± 0.4 [12]LS LR | 3.5 ± 0.5 [12]LS LR | 1.92 ± 0.24 [9]DR LS LR | 6.05 ± 0.05 [9]LS |
Leth/Swank | 38.8 ± 2.6 [10]DS DR LR | 23.0 ± 1.8 [10]DS DR | 15.8 ± 1.0 [10]DS DR LR | 1.01 ± 0.03 [10]DS | 5.60 ± 0.04 [10]DS DR LR |
Dros/Reedy | 10.8 ± 1.2 [10]LS LR | 6.5 ± 0.8 [10]LS LR | 4.3 ± 0.6 [10]LS LR | 1.29 ± 0.19 [9]DS | 6.09 ± 0.14 [9]LS |
Leth/Reedy | 52.5 ± 1.1 [10]DS DR LS | 21.4 ± 0.9 [10]DS DR | 31.1 ± 1.1 [10]DS DR LS | 1.14 ± 0.03 [10]DS | 6.29 ± 0.04 [10]LS |
All values are means ± SE. ASA, total active stretch tension (pCa 5.0); PSA, passive stretch tension (pCa 8.0); FSA, corrected active stretch tension (FSA = ASA − PSA). Graphs of the relationship between stretch-activated tension, FSA, and calcium concentration from individual fibers were fit with the Hill equation to obtain the average Hill coefficient and pCa50 values. Square brackets indicate number of fibers tested. Superscript letters indicate statistical difference between the four sets of fibers based on one-way ANOVA with Holm-Sidak pairwise multiple comparisons, P < 0.05. DS = Drosophila fibers bathed in Swank solutions; DR = Drosophila fibers bathed in Reedy solutions; LS = Lethocerus fibers bathed in Swank solutions; LR = Lethocerus fibers bathed in Reedy solutions.