Fig. 7.

Effects of siRNA-mediated silencing of PCSK7, PCSK9, and S1P on PRR cleavage induced by BSA in HK-2 cells. The cells were transfected with siRNA against PCSK7, PCSK9, or S1P for 48 h and then treated with 20 mg/ml BSA for 24 h. A and B: immunoblotting of fPRR and sPRR. Shown are representative blots and quantitative densitometry analysis. C and D: ELISA detection of medium sPRR. The values are normalized by protein content. *P < 0.05 vs. CTR; #P < 0.05 vs. BSA; NS, nonsignificance vs. BSA. Data are means ± SD; n = 6/group. C and D values are presented as the median (central line), interquartile range (box), and range (whiskers).