Table 2.
Primer sequences for real-time PCR
| Target Gene | Primer Sequence |
|---|---|
| Human-GAPDH, F | TCATTGACCTCAACTACATG |
| Human-GAPDH, R | CAAAGTTGTCATGGATGACC |
| Human-PRR, F | CAGACGTGGCTGCATTGTCC |
| Human-PRR, R | CTGGGGGTAGAGCCAGTTTGTT |
| Human-ACE, F | GGAGGAATATGACCGGACATCC |
| Human-ACE, R | TGGTTGGCTATTTGCATGTTCTT |
| Human-Furin, F | GCAATAATGGTCCCCATCC |
| Human-Furin, R | GCCTCCTCCTTCCAATATCC |
| Human-ADAM19, F | AAGTACCATGACAACGCCCAAT |
| Human-ADAM19, R | CATTCTCGGAGTGGTCCATGT |
| Human-PCSK1, F | CTGGATGGCATTGTGACGGAT |
| Human-PCSK1, R | GCCCCAGCTTGCACTGTAAA |
| Human-PCSK2, F | GGGAAAGGTGTTACCATTGGAA |
| Human-PCSK2, R | CCAGTCATCTGTGTACCGAGG |
| Human-PCSK4, F | CACGCAAATTCGGCTTCGTC |
| Human-PCSK4, R | ACGACAGAGCGTTTCACCC |
| Human-PCSK5, F | CCAACCACTGGGCAGTCAAA |
| Human-PCSK5, R | CTGACCTTTTAATCGTCCTGCT |
| Human-PCSK6, F | CGCAGGCCCTTTACTTCAAC |
| Human-PCSK6, R | CGGCAGCGACTGTTCTTGT |
| Human-PCSK7, F | GCAGCGTCCACTTCAACGA |
| Human-PCSK7, R | GCCCAGTCACATTGCGTTC |
| Human-PCSK9, F | AGACCCACCTCTCGCAGTC |
| Human-PCSK9, R | GGAGTCCTCCTCGATGTAGTC |
| Human-S1P, F | ACCTCGAAACAATCCATCCAGT |
| Human-S1P, R | ACTTGAGGGAACGAAAGACTTTT |
| Human-TGF-β1, F | TCCACCTGCAAGACTATCGAC |
| Human-TGF-β1, R | GAGGTATCGCCAGGAATTGTT |
| Human-IL-6, F | ACTCACCTCTTCAGAACGAATTG |
| Human-IL-6, R | CCATCTTTGGAAGGTTCAGGTTG |
| Human-IL-8, F | TTTTGCCAAGGAGTGCTAAAGA |
| Human-IL-8, R | AACCCTCTGCACCCAGTTTTC |
F, forward; R, reverse; PRR (ATP6AP2), prorenin receptor; ACE, angiotensin-converting enzyme; ADAM19, a disintegrin and metalloproteinase domain 19; PCSK1/2/4/5/6/7/9, Homo sapiens proprotein convertase subtilisin/kexin type 1/2/4/5/6/7/9; S1P, also known as SKI-1, membrane-bound transcription factor peptidase, site-1; TGF-β1: transforming growth factor-β1.