. 2017 Oct 17;16(3):784–796. doi: 10.1111/pbi.12828

Table 1.

Sterol‐related intermediate, sterol, α‐tocopherol and carotenoid profiles of tomato HMGS‐OE fruits [μg/g dry weight (DW) for sterols and α‐tocopherol, mg/g DW for carotenoids]

Sterols	pSa13	430	445	622	625
Squalene	25.2 ± 0.55	24.5 ± 1.49	45.7 ± 0.71 ^a	58.9 ± 1.98 ^a ^, ^b	99.1 ± 2.04 ^a ^, ^b
Cycloartenol	33.8 ± 2.79	94.9 ± 5.22 ^a	68.2 ± 1.40 ^a	76.3 ± 0.60 ^a	128.4 ± 8.39 ^a ^, ^b
24‐Methylene‐cycloartanol	83.2 ± 6.84	74.5 ± 1.16	159.8 ± 4.84 ^a	103.4 ± 0.05 ^a	89.0 ± 1.49
Cycloeucalenol	38.1 ± 1.42	61.3 ± 3.08 ^a	54.5 ± 1.65 ^a	68.9 ± 1.00 ^a	83.1 ± 5.68 ^a ^, ^b
24‐Methylene‐lophenol	52.2 ± 1.69	93.0 ± 4.09 ^a	112.7 ± 2.18 ^a	86.7 ± 0.75 ^a	122.1 ± 6.37
Campesterol	78.2 ± 2.8	98.0 ± 2.3 ^a	94.2 ± 2.3 ^a	152.8 ± 7.7 ^a ^, ^b	152.2 ± 1.5 ^a ^, ^b
β‐Sitosterol	86.2 ± 2.5	119.2 ± 1.7 ^a	123.2 ± 3.5 ^a	161.3 ± 4.3 ^a ^, ^b	183.3 ± 4.8 ^a ^, ^b
Stigmasterol	105.2 ± 4.5	169.9 ± 1.5 ^a	140.9 ± 2.5 ^a	155.4 ± 1.9 ^a	183.5 ± 2.6 ^a ^, ^b
Δ^7,22‐Ergostadienol	43.8 ± 1.6	109.2 ± 1.2 ^a	88.5 ± 2.3 ^a	86.7 ± 1.6 ^a	123.5 ± 2.1 ^a ^, ^b
Total sterol	546.0 ± 12.3	844.5 ± 10.9 ^a	887.6 ± 10.7 ^a	950.4 ± 10.0 ^a ^, ^b	1164.2 ± 17.5 ^a ^, ^b
α‐Tocopherol	27.5 ± 3.2	176.4 ± 4.4 ^a	164.7 ± 6.2 ^a	138.6 ± 13.4 ^a	187.5 ± 3.6 ^a
Lycopene	3.0 ± 0.1	10.7 ± 1.2 ^a	9.3 ± 0.5 ^a	6.7 ± 0.7 ^a ^, ^b	6.0 ± 0.2 ^a ^, ^b
β‐Carotene	1.5 ± 0.06	6.7 ± 0.09 ^a	3.8 ± 0.1 ^a	3.5 ± 0.3 ^a	4.5 ± 0.09 ^a
Total carotenoids	4.5 ± 0.16	17.4 ± 1.29 ^a	13.1 ± 0.6 ^a	10.2 ± 1.0 ^a ^, ^b	10.5 ± 0.29 ^a ^, ^b

Two independent lines for each OE genotype were analysed. For OE‐wtBjHMGS1, lines 430 and 445 were tested. For OE‐S359A, lines 622 and 625 were tested. ^aIndicates significant difference (P < 0.01 by the Student's t‐test) between HMGS‐OE and the vector (pSa13)‐transformed control; ^bIndicates significant difference (P < 0.01 by the Student's t‐test) between OE‐wtBjHMGS1 and OE‐S359A. Total sterol content was calculated from the contents of all the components in the above table. Values are mean ± SD, n = 8 for sterol and 6 for carotenoid determination.