Feasibility, sensitivity and selectivity of the NaA43 DzCHA probe. (A) Fluorescence spectra of the DzCHA probe with control H1 (black), non-cleavable NaA43 DzCHA probe (blue), DzCHA probe in buffer without Na+ (pink), and DzCHA probe in buffer with 100 mM Na+ (green). (B) Fluorescence signal increase of the sensor over different concentration of Na+, ranging from 0, 0.1, 0.5, 1, 4, 6, 9, 12, 15, 20, 30, 40, 100 mM. (C) Plot of fluorescence intensity versus Na+ concentration. The inset shows a magnification of the linear region. Error bars represent the SD from three independent experiments. (D) Response of the probe to different competing metal ions. The mono-, di-, and trivalent metal ion is 30 mM, 1 mM, and 0.1 mM, respectively.