Fig. 5.

Oleate specifically increases SREBP-1 expression and nuclear localization. A: SCD1 (red) and SREBP-1 (green) expression was evaluated by immunofluorescence on HepG2 cells transfected with negative control (Ctrl) or SCD1-targeting siRNA (siSCD1 or siR), or incubated with 1 μM SCD1 inhibitor A939572 (inh.). Cells were treated with 100 μM oleate or palmitoleate (Palm.). DAPI (blue) was used to visualize nuclei. Bars = 20 μm. The SREBP-1 signal per cell (B and D), as well as the SREBP-1 nuclear proportion (C and E), was measured in all experimental conditions. Each histogram presents the means ± SD of 3 independent experiments. *P < 0.05 and **P < 0.002.