Fig. 6.

The SREBP-1 protein is specifically modified by oleate. SREBP-1 was immunoprecipitated from protein extracts of HepG2 cells previously incubated 24 h with [³H]H₂O, [³H]oleate, or [³H]palmitoleate (palm.). Immunoprecipitates (IP) were submitted to denaturing gel electrophoresis to separate precursor and mature SREBP-1 (pSREBP-1 and mSREBP-1). Incorporated radioactivity was measured in isolated mSREBP-1 protein bands and presented relative to nonradioactive control (Ctrl). WB, Western blot. The histogram presents the means ± SD of 3 independent experiments. ***P < 0.001.