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. 2017 Aug 29;313(6):E710–E720. doi: 10.1152/ajpendo.00151.2017

Fig. 7.

Fig. 7.

SREBP-1 expression and de novo lipogenesis are partially restored in the liver of Scd1 knockout mice specifically producing oleate. Livers of control wild-type mice (Ctrl), Scd1 knockout mice (GKO), and mice specifically producing oleate or palmitoleate (GLS5 and GLS3, respectively) were used to evaluate the importance of SCD1 MUFA products in SREBP-1 expression and de novo lipogenesis. A: SREBP-1 expression, for both precursor (pSREBP-1) and mature (mSREBP-1) forms, in total protein extracts was analyzed by Western blotting, in triplicate. Protein levels evaluated by densitometry were normalized against α-tubulin and presented relative to wild-type control. B: expression of key genes implicated in DNL (Srebp-1, Acc, and Fas) were measured by qPCR, normalized against Hprt and presented relative to wild-type control. C: protein expression of pAMPK, AMPK, pACC, and ACC was measured by Western blotting, in triplicate. Protein levels measured by densitometry were normalized against α-tubulin, used to calculate the phosphorylated/total ratio, and presented relative to wild-type control. Each histogram presents the means ± SD of 5 tissue measurements. *P < 0.05, **P < 0.002, and ***P < 0.001.