Table 1.
Species/Gene* | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
---|---|---|
Mouse | ||
Srebp-1 (Srebf1) | CCTGCCTTCAGGCTTCTCAGG | GAGGCCAAGCTTTGGACCTGG |
Fas (Fasn) | ATTGCATCAAGCAAGTGCAG | GAGCCGTCAAACAGGAAGAG |
Acc (Acaca) | TGAAGGGCTACCTCTAATG | TCACAACCCAAGAACCAC |
Hprt | TCAGTCAACGGGGGACATAAA | GGGGCTGTACTGCTTAACCAG |
Human | ||
SREBP-1 (SREBF1) | ACAGTGACTTCCCTGGCCTAT | GCATGGACGGGTACATCTTCAA |
HPRT1 | CCTGGCGTCGTGATTAGTGAT | AGACGTTCAGTCCTGTCCATAA |
qPCR, quantitative reverse-transcription-polymerase chain reaction; SREBP-1, sterol receptor element binding protein-1; FAS, fatty acid synthase; ACC, acetyl-CoA carboxylase; HPRT1, hypoxanthine phosphoribosyltransferase 1.
Official gene names are provided in parentheses when different from the common name used in this study.