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. 2017 Aug 29;313(6):E710–E720. doi: 10.1152/ajpendo.00151.2017

Table 1.

Primer sequences for mouse and human genes used in qPCR

Species/Gene* Forward Primer (5′–3′) Reverse Primer (5′–3′)
Mouse
    Srebp-1 (Srebf1) CCTGCCTTCAGGCTTCTCAGG GAGGCCAAGCTTTGGACCTGG
    Fas (Fasn) ATTGCATCAAGCAAGTGCAG GAGCCGTCAAACAGGAAGAG
    Acc (Acaca) TGAAGGGCTACCTCTAATG TCACAACCCAAGAACCAC
    Hprt TCAGTCAACGGGGGACATAAA GGGGCTGTACTGCTTAACCAG
Human
    SREBP-1 (SREBF1) ACAGTGACTTCCCTGGCCTAT GCATGGACGGGTACATCTTCAA
    HPRT1 CCTGGCGTCGTGATTAGTGAT AGACGTTCAGTCCTGTCCATAA

qPCR, quantitative reverse-transcription-polymerase chain reaction; SREBP-1, sterol receptor element binding protein-1; FAS, fatty acid synthase; ACC, acetyl-CoA carboxylase; HPRT1, hypoxanthine phosphoribosyltransferase 1.

*

Official gene names are provided in parentheses when different from the common name used in this study.