Fig. 3.

2-Bromopalmitate (2-BP) prevents epithelial barrier dysfunction and improves TNF-α-IFN-γ-mediated increase in total protein palmitoylation. A: mouse intestinal epithelial cells (MIEpCs) were grown to 2 days postconfluence on electric cell-substrate impedance-sensing arrays and then treated with 100 ng/ml TNF-α and 500 U/ml IFN-γ; resistance measurements were recorded every 90 s. B: MIEpCs were grown to 2 days postconfluence on transwell inserts and then treated with TNF-α-IFN-γ for the indicated time points. FITC-labeled dextran (4 kDa; 2.5 mg/ml) was added to the luminal compartment, and abluminal samples were read on a fluorometer. C: MIEpCs were pretreated with vehicle control or 2-BP for 3 h and then TNF-α-IFN-γ for 24 h. Lysates then underwent acyl-biotin exchange assay to identify total palmitoylation. *P < 0.05 compared with control, #P < 0.05 compared with TNF-α-IFN-γ.