Fig. 4.

Effect of transient cotransfection of mimic-423-5p with pmirGLO-3′-UTR-hRFVT3 on luciferase activity Caco-2 (A) and HuTu-80 (B) cells. Cells were transiently cotransfected with pmirGLO-vector or the pmirGLO-3'-UTR-hRFVT3 construct along with miRNA mimic (negative control mimic, mimic-423-5p, 50 nM, 48 h) and were assayed for luciferase activity, as described in materials and methods. Data are expressed as means ± SE of three independent experiments. #pmirGLO-3'-UTR-hRFVT3+mimic-423-5p is significantly decreased in the luciferase activity compared with pmirGLO-3'-UTR-hRFVT3+mimic-ve (**P < 0.01).