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Effect of mutating the miR-423-5p putative site in the 3′-UTR-hRFVT3 on luciferase activity in Caco-2 cells. Cells were cotransfected with miR-423-5p mimic and pmirGLO-3′-UTR-hRFVT3 or mutated construct (pmirGLO-3′-UTR-hRFVT3). Luciferase activities were measured and normalized with respective Renilla luciferase activities. Data are expressed as means ± SE of at least three independent experiments (**P < 0.01).
