Table 1.
List of primers used in the study
Primer | Sequence |
---|---|
3′-UTR-hRFVT3 in pmirGLO vector | Forward: 5′-CCGCTCGAGCTGCACTGTCCAGCCTAG-3′ |
Reverse: 5′-CGCGTCGACAAGCAGGGAATGCCT-3′ | |
Real-time PCR primers hRFVT3 | |
h-RFVT3-RT | Forward: 5′-CTGGTCTGCGTCTTCGGAATG-3′ |
Reverse: 5′-ACCACCGTGAGGTAGGAGG-3′ | |
m-RFVT3-RT | Forward: 5′-GGATCAGTGGAAGCCAGTG-3′ |
Reverse: 5′-GACCTGTTAGGCAGGAAGATG-3′ | |
hβ-actin-RT | Forward: 5′-CATCCTGCGTCTGGACCT-3′ |
Reverse: 5′-TAATGTCACGCACGATTTCC-3′ | |
mβ-actin-RT | Forward: 5′-ATCCTCTTCCTCCCTGGA-3′ |
Reverse: 5′-TTCATGGATGCCACAGGA-3′ | |
Mutant primers | Forward: 5′-GCCCCTGCCCTCTCTCCTTTACTAATCAGTCTCACAGAGGG-3′ |
Reverse:5′-CCCTCTGTGAGACTGATTAGTAAAGGAGAGAGGGCAGGGGC-3′ |
Restriction sites are underlined, and mutated sites are in boldface italics.