Fig. 2.

Addition of DAS or NAC does not prevent the ethanol-induced impairment of secretion. A: WIF-B cells were treated in the absence or presence of 50 mM ethanol (EtOH) for 72 h as indicated. B: cells were treated with 100 μM DAS in the absence or presence of ethanol. C: cells were treated with 5 mM NAC were incubated in the absence or presence of ethanol. Cells were rinsed 5 times with prewarmed serum-free medium and then reincubated in serum-free medium. At 0, 5, 15, and 30 min after reincubation, aliquots of media were collected and analyzed for albumin secretion by immunoblotting (left). The percent albumin secreted was calculated from densitometric analysis of immunoreactive bands and plotted in the graphs shown on the right. Values are expressed as the means ± SE from 3 independent experiments.