Fig. 5.

Addition of DAS or NAC prevents the ethanol-induced impairment of Jak2 phosphorylation. A: WIF-B cells were treated in the absence or presence of 50 mM ethanol in the additional presence of 100 μM DAS or 5 mM NAC as indicated. Samples were immunoblotted for total Jak2 expression. B: WIF-B cells were treated in the absence or presence of 50 mM ethanol (EtOH) for 72 h as indicated. C: cells were treated with 100 μM DAS were treated in the absence or presence of ethanol. D: cells were treated with 5 mM NAC were incubated in the absence or presence of ethanol. Cells were incubated for up to 15 min with GH and lysates immunoblotted for phospho-Jak2 (pJak2) (left). Immunoreactivity was measured by densitometry, normalized to total Jak2 levels and plotted as the percentage of maximal phosphorylation (right). Values represent the means ± SE from 3 independent experiments.