Fig. 8.

Only CYP2E1-expressing cells display ethanol-induced impairments in Jak2 and STAT5B activation. A: E47 or VA-13 cells were treated in the absence or presence of 50 mM ethanol for 24 h. Cells were incubated for up to 15 min with GH and lysates immunoblotted for total Jak2 and STAT5B as indicated. A representative immunoblot from at least 3 independent experiments is shown for each. E47 (B and C) and VA-13 (D and E) cells were treated in the absence or presence of 50 mM ethanol (EtOH) for 24 h as indicated. Cells were incubated for up to 15 min with GH and lysates immunoblotted for phospho-Jak2 (B and D) or phospho-STAT5B as indicated (C and E). Immunoreactivity was measured by densitometry, values were normalized to total Jak2 or STAT5B levels, and maximum values were set to 100%. Values represent the means ± SE from at least 3 independent experiments in B–E. Due to variability in peak activation time, one representative immunoblot and graph out of 4 independent experiments are shown in C.