Fig. 4.
The conditional ablation of glial connexin-43 exacerbates the effects of NH4Cl on neuromuscular transmission. Neurogenic contractions (A and B) and relaxations (C-D) of the mouse ileum driven by EFS (20 V, 0.3 ms, 20 Hz) in hGFAP::CreERT2+/−/Cx43f/f mice. NH4Cl was more efficacious at potentiating neurogenic contractions (A and B) and diminishing relaxations (C and D) in hGFAP::CreERT2+/−/Cx43f/f mice than wild-type (WT) mice (see Fig. 1). MSO (1 mM) did not further potentiate the effects of NH4Cl and PAPA NONOate (100 μM) partially antagonized the effects of NH4Cl. Data are means ± SE and were analyzed by two-way ANOVA and Bonferonni’s post hoc test. *P < 0.05, **P < 0.01, #****P < 0.001, compared with control; $****P < 0.001, compared with NH4 or MSO (n = 5 in each group).