Fig. 1.

OGG1 expression exacerbates pollen-induced allergic inflammation. A: OGG1 silencing in lungs decreases the accumulation of eosinophils (gray bars) compared with those expressing OGG1 (open bars). Sensitized OGG1-expressing (OGG1^P) and OGG1-deficient (OGG1^D) mice were challenged with intrinsic NADPH oxidase of RWPE (RWPE^NOX), heat-inactivated RWPE (RWPE^HI), RWPE^NOX+8-oxoG, or RWPE^HI+8-oxoG. Seventy-two hours later, lungs were lavaged, and the numbers of BALF eosinophils were determined. B and C: expression of OGG1 at protein (B) and RNA (C) levels in the lungs of sensitized mice prior to and after OGG1 silencing. OGG1 silencing in airways is described in materials and methods. Protein and RNA levels in lungs of OGG1^D and OGG1^P mice were determined 72 h after RWPE challenge. D: RWPE^NOX increases cellular ROS levels in cultured airway epithelial (AML-12) cells (open bars). RWPE^HI had no effect on cellular reactive ROS levels (gray bars). Changes in ROS levels were determined by oxidation of H₂DCF (see materials and methods). E and F: RWPE^NOX challenge increased the protein carbonyl (E) and genomic 8-oxoG (F) levels in lungs of OGG1^P and OGG1^D. MWM, molecular weight marker. Data are presented as means ± SE of six individual mice. ***P < 0.001.