Fig. 5.

TREK-1/TREK-2/TRAAK-deficient lungs contained lower ATP-binding cassette, subfamily A, member 3 (ABCA3)/lamellar body levels than WT controls at baseline but not after HO exposure. At baseline, triple KO lungs contain lower ABCA3/lamellar body levels than WT controls, but HO exposure increased ABCA3/lamellar body levels in both WT control and triple KO lungs as shown by representative Western blot (A), semiquantitative optical densitometry measurements (B), and confocal immunofluorescence microscopy (C). ABCA3 protein is shown in red, and nuclei were counterstained in blue with DAPI (n = 5 mice per each group; *comparison of WT control and triple KO mice within 1 treatment group; ^#compared with untreated WT control mice; ^compared with untreated triple KO mice).