Fig. 7.

Cytokine and zonula occludens-1 (ZO1) expression in TREK-1/TREK-2/TRAAK-deficient lungs. A: Representative images of IL-6, monocyte chemotactic protein-1 (MCP-1), and IL-18 mRNA levels detected in whole lung homogenates of WT control and triple KO lungs by conventional PCR. B: IL-22 levels measured in whole lung homogenates of WT control and triple KO mice by semiquantitative, real-time PCR (data are depicted as fold-change in gene expression normalized to WT RA). C: IL-10 levels in BAL fluid of WT control and triple KO lungs measured by ELISA. D: similar levels of ZO1 expression by confocal immunofluorescent microscopy in WT control and triple KO lung slices at baseline and after 72-h HO exposure (n = 5–6 mice per group; *comparison of WT control and triple KO mice within 2 treatment groups; ^compared with untreated triple KO mice).