Fig. 1.
Inward current induced by nipecotic acid (NA). A–G: examples of NA-activated currents recorded in whole cell mode from an SCN neuron voltage-clamped at −60 mV. A: NA induced an inward tonic current and reduced the amplitude of synaptic currents. B: gabazine (Gbz; 10 μM) reversely inhibited the GABAAR-mediated tonic current induced by NA. C–E: increase of the tonic current and decrease of sGPSC amplitude during application of NA in concentrations of 1 (C), 2 (D), and 5 mM (E) (successively: control, NA application, and washout). F: transient current during 50 mM NA application (extended scale). Inset: recordings 1–4 demonstrate a reduction of the sGPSC amplitude during activation of the transient current. G: the NA (40 mM)-activated inward current (left) was occluded by the tonic current induced by coapplication of SKF-89976A and SNAP-5114 (SKF+SNAP, 100 µM each; right).