Fig. 4.

Concentration dependence of GABA_AR-mediated tonic current on the GAT1 and GAT3 inhibitors. A: concentration dependence of the inward tonic current activated by the GAT1 inhibitor SKF-89976A (I_∆ = I_(SKF+SNAP) − I_SNAP, recording solution contained 20 µM SNAP-5114; whole cell recording from cerebral cortex; EC₅₀ = 32.6 ± 11.5 µM, n = 26 cells). B: concentration-response curve for GAT3 inhibitor SNAP-5114 (I_∆ = I_(SNAP+SKF) − I_SKF, recording solution contained 10 µM SKF-89976A; recording from SCN; EC₅₀ = 79.0 ± 19.4 µM, n = 39 cells). On the y-axis, I_∆ (inward tonic current, in pA) is shown as positive numbers (means ± SE).