Figure 1.

Conservation of MAPK phosphosites in PIN proteins in land plants and their co‐occurrence with PID phosphosites. Arabidopsis, rice and Physcomitrella patens members of the UNIPROT PIN auxin efflux protein family were aligned using the MUSCLE algorithm. The regions shown correspond to G223‐D295 (A) and G319‐Y394 (B) in the hydrophilic loop of PIN1. MAPK and PID phosphorylation sites and preferred MAPK phosphorylation flanking residues are highlighted in red, black and grey backgrounds respectively. Positions of potential MAPK sites in the full‐length PIN1 protein are indicated below. Full‐length alignment is provided in Fig. S1.