Figure 5.

Dense‐core vesicle (DCV) fusion events are less frequent, but fusion probability is normal in the absence of Cgs. (a) Example of a neuropeptide Y (NPY)‐pHluorin release event (arrowhead) in a control neurite and the unquenching of all the DCVs present in the area upon NH ₄Cl puff (arrows). Scale bar: 2 μm. (b) Averaged frequency distribution of the events against the time of recording. SEM plotted as dotted line. The stimulation is shown in gray (16 bursts of 50 action potentials at 50 Hz). (c) Averaged cumulated fusion events per neuron. (d) Normalized cumulated fusion events. (e) Quantification of the total number of fusion events per neuron. (f) Total DCV pool per neuron calculated from NH ₄Cl puff signal. (g) Fusion probability of DCVs of both genotypes. (h) NPY‐pHluorin puncta intensity in the NH ₄Cl puff. (Data are shown as mean ± SEM. MW‐U test *p = 0.0477. Control n = 29 neurons and CgA/B^−/− n = 36 neurons from four independent cultures. (e, g, and h) MW‐U test. (f) Student's t‐test.