Table 1.

Oligonucleotides and sgRNA used in the study

Name	Sequence	Description
P1	TATAGAATTCACCATGcatcaccatcaccatcacGGTGGCGGTATGTCTGCCGCTGTCGCAT	gluF ORF amplification with His‐tag, EcoRI site, fwd primer
P2	TATAGGATCCTTATCTACCCATCC	gluF ORF amplification with His‐tag, BamHI site, rev primer
P3	GAGCTATCAGTGAGAACGAT	gluF 5′, 609 bp up from the strart codon, fwd primer
P4	ACTCTGACTCATCTCGCGCC	gluF 3′, 600 bp down from gRNA PAM sequence, rev primer
P5	TATATAGAGCTCCACCATGTCGGCAGCAGTTGCGTC	Amplification of gluF ORF, SacI flank, fwd primer
P6	TATATACCCGGGTCACCGTCCCATCCAACCGC	Amplification of gluF ORF, XmaI flank, rev primer
gluF sgRNA	GCGTCGCTATTTTCCCTCAG	gluF sgRNA protospacer sequence